✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Middleburg, FL

With 36 testing centers in the Middleburg area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

36 Drug-Testing Centers in Middleburg

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

1708 Blanding Blvd

3.14 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
1708 Blanding Blvd
Middleburg, FL 32068

Onsite Only

9.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Onsite Only
Fleming Island, FL 32003

1680 Eagle Harbor Parkway, Suite B

9.66 mi
🕑 Testing Hours: Mon–Fri: 12:00 PM-2:00 PM • Sat: 8:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
1680 Eagle Harbor Parkway, Suite B
Orange Park, FL 32003

2140 Kingsley Ave

9.67 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
2140 Kingsley Ave
Orange Park, FL 32073

2020 Kingsley Ave Ste A

9.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2020 Kingsley Ave Ste A
Orange Park, FL 32073

1895 Kingsley Ave Ste 803

9.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1895 Kingsley Ave Ste 803
Orange Park, FL 32073

1563 Kingsley Ave Ste 102

10.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1563 Kingsley Ave Ste 102
Orange Park, FL 32073

1465 Kingsley Ave Ste 1401,

10.48 mi
🕑 Testing Hours: Mon–Fri: 12:00 PM-2:00 PM✓ Walk-in with Electronic Donor Pass
1465 Kingsley Ave Ste 1401,
Orange Park, FL 32073

3185 Highway 17

11.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3185 Highway 17
Green Cove Springs, FL 32043

705 Ferris Street

11.95 mi
🕑 Testing Hours: M-Th 8:00 AM-5:00 PM • F 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
705 Ferris Street
Green Cove Springs, FL 32043

12303 San Jose Blvd

14.65 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
12303 San Jose Blvd
Jacksonville, FL 32223

5501 Roosevelt Blvd

15.72 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5501 Roosevelt Blvd
Jacksonville, FL 32244

7963 Normandy Blvd

16.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7963 Normandy Blvd
Jacksonville, FL 32221

6671 Hyde Grove Ave

16.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6671 Hyde Grove Ave
Jacksonville, FL 32210

1584 Normandy Village Parkway Ste 32

16.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1584 Normandy Village Parkway Ste 32
Jacksonville, FL 32221

4616 San Juan Ave

16.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4616 San Juan Ave
Jacksonville, FL 32210

1912 Hamilton St Ste 103

16.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1912 Hamilton St Ste 103
Jacksonville, FL 32210

9826 San Jose Blvd

16.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9826 San Jose Blvd
Jacksonville, FL 32257

9857 Old Saint Augustine Rd Ste 4

17.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9857 Old Saint Augustine Rd Ste 4
Jacksonville, FL 32257

1610 Blanding Blvd

17.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1610 Blanding Blvd
Jacksonville, FL 32210

345 W Madison St

17.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
345 W Madison St
Starke, FL 32091

5964 Normandy Blvd

17.73 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
5964 Normandy Blvd
Jacksonville, FL 32205

231 Airline Way

18.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
231 Airline Way
Jacksonville, FL 32217

4221 Baymeadows Rd Ste 6

18.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4221 Baymeadows Rd Ste 6
Jacksonville, FL 32217

1550 S Water St

18.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1550 S Water St
Starke, FL 32091

4498 Hendricks Ave

18.96 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
4498 Hendricks Ave
Jacksonville, FL 32207

7033 Commonwealth Ave Ste 8

19.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7033 Commonwealth Ave Ste 8
Jacksonville, FL 32220

9143 Philips Hwy Ste 495

19.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9143 Philips Hwy Ste 495
Jacksonville, FL 32256

2063 Oak St

19.90 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-4:00 PM • Sat: 10:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
2063 Oak St
Jacksonville, FL 32204

837 Edgewood Ave N

19.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
837 Edgewood Ave N
Jacksonville, FL 32254

7933 Baymeadows Way Ste 3

20.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7933 Baymeadows Way Ste 3
Jacksonville, FL 32256

4933-1 University Blvd W

20.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4933-1 University Blvd W
Jacksonville, FL 32216

4237 Salisbury Rd Ste 304

20.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4237 Salisbury Rd Ste 304
Jacksonville, FL 32216

6639 Southpoint Pkwy Ste 106

20.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6639 Southpoint Pkwy Ste 106
Jacksonville, FL 32216

4063 Salisbury Rd Ste 205

20.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4063 Salisbury Rd Ste 205
Jacksonville, FL 32216

1325 San Marco Blvd Ste 301

20.65 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1325 San Marco Blvd Ste 301
Jacksonville, FL 32207

Employment, DOT, Court Ordered, Probation, Family

At Accredited Drug Testing, we provide an extensive range of drug and alcohol screenings in the Middleburg, FL area, boasting 36 centers. Our services include both DOT and non-DOT urine tests, breath alcohol tests, EtG alcohol assessments, and hair drug analyses for personal, professional, and legal requirements. Quick results testing and SAMSA certified lab evaluations are available in Middleburg, FL, with same-day service possible. Many testing centers are conveniently located near your residence or workplace. Additional services comprise Occupational Health Testing, Clinical Testing, and Background Checks.

Dial (800) 221-4291 or enroll online at any time. Pick your preferred test and select a nearby location—testing options are available for yourself, your workforce, or someone else. Scheduling a test is swift and straightforward by contacting our scheduling team or organizing your test online anytime. Our efficient and accessible process makes setting up drug testing near Middleburg a breeze.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Middleburg, FL

1

Order Online or Call

Select your test & search nearby Middleburg collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Middleburg, FL Drug Testing

Drug Testing Services Available at Our Middleburg, FL Labs:

At our Middleburg drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Florida Drug Testing Laws & Compliance in Middleburg

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Middleburg, FL adhering strictly to Fla. Stat. § 440.102, federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
Fla. Stat. § 440.102
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Prohibited
Workplace Rights
Zero-Tolerance Protected

Florida Statute 440.102 establishes Drug-Free Workplace Program standards, providing premium discounts on worker compensation insurance for verified testing procedures.

Under Fla. Stat. § 440.102, employers in Middleburg may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Prohibited in Florida), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Middleburg, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Middleburg and Florida.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Middleburg, FL

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Middleburg to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Middleburg Neighborhoods Covered:

● Downtown Middleburg ● Central Business District ● North Middleburg Commercial Corridor ● South Middleburg Industrial Park ● East Middleburg Metro Area ● West Middleburg Trade Center ● Medical & Healthcare District ● Airport / Logistics Corridor

Major Employment & Commercial Hubs Served:

Middleburg Main Street Commercial District • Middleburg Regional Trade Center • County Industrial Parkway • Metro Healthcare & Occupational Medical Corridor

Located anywhere in Middleburg? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Middleburg

Asbury Lake • Middleburg-clay Hill • Doctors Inlet • Russell • Penney Farms • Lakeside • Oakleaf Plantation • Fleming Island

Middleburg, FL Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Middleburg.

Test Type & Local Panel Detection Window Common Use Cases in Middleburg Turnaround Time
5-Panel Urine Screen in Middleburg →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Middleburg →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Middleburg →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Middleburg →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Middleburg →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Middleburg →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Middleburg, FL

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Middleburg.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Middleburg facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Middleburg, FL

Need immediate walk-in service in Middleburg? View our Walk-In Drug Testing Near Me in Middleburg, FL facility guide or order an instant electronic donor pass online.

Middleburg Drug Testing

Quickly find trusted local drug testing centers in Middleburg, FL — fast, convenient, and reliable every time!

Middleburg DOT Drug Testing

Quickly find a local DOT drug testing center in Middleburg, FL — fast, reliable, convenient nationwide service!

Middleburg DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Middleburg, FL.

Middleburg Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Middleburg, FL.v

Middleburg Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Middleburg, FL locations—results you can trust, every time!

Middleburg Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Middleburg, FL.

Middleburg Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Middleburg, FL.

Middleburg 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Middleburg, FL employers and individuals nationwide.

Middleburg 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Middleburg, FL workplace and personal testing needs.

Middleburg On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Middleburg, FL workplace compliant.

Middleburg DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Middleburg, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Florida, workplace drug testing is governed by statutory guidelines under Fla. Stat. § 440.102. Private employers generally operate under a Drug-Free Workplace Program under Fla. Stat. § 440.102, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Florida who implement a certified Drug-Free Workplace Program in compliance with Fla. Stat. § 440.102 can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Florida state law compliance standards, state statutes does not require accommodation of medical marijuana in workplace. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 36 certified collection sites in the Middleburg area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Middleburg, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

A very nice company

A very nice company of expertise. The staff will go above to help you out of your situation in a timely matter 👏

Tam Jack - 2/19/2025

Kind and very helpful

Called around for hours and posted from company to person and got nowhere and had to fight to get a refund die to there miss information, well save time and sanity Acredited is the ONLY place the person was kind and very helpful, said what I needed and Faith the lady I dealt with made it simple and she did above and beyond everything I needed, as I'm going through a devorse this was a great help and made my day 100 stars from me.

Steven Harrison - 12/19/2024

Thank You!!

I needed a quick drug test for visitation with my children. I called at 6am they answered and set me up with a place a couple miles from my house to go get the test done, that was on Wednesday, I had the results on Friday afternoon and was able to see my kids on Saturday. Thank you for the help!

Mark Broadhead - 2/12/2025

Super helpful

Customer service was surprisingly super helpful and so nice. She was able to get me in at a local location right away the same day. She helped me figure out exactly what I needed. Very impressed.

Brook - 9/19/2024

Sweet and helpful

Trish was amazing and got me through the sytem very fast and swift. I had a hard time hearing her a couple of times, but she was super sweet and helpful throughout the process. Highly recommend her!

Sophia Schutze - 6/19/2024

Quick, knowledgeable and courteous

Quick, knowledgeable and courteous Janelle worked diligently to support me. I am recommending their services to anyone looking.

Greensboro Joseph - 11/19/2024

ADT is truly the best

Where do I start?!! ADT is truly the best!! I had the pleasure of speaking with Tori today! She was excellent, professional and truly went above and beyond in her kind, compassionate care and commitment to providing me with the best service possible. I was in such a time crunch, but I was able to call, order the test, pay for it over the phone and get the test I needed within the hour. Not only did this put my mind at ease, they saved my job!! I am forever grateful for this team of hard workers, that care, with such great work ethics! Thank you from the very bottom of my heart!!! If you need a drug, alcohol, etc… screening, this team will be your lifesavers!!

Sarah Patterson - 4/8/2025

Extremely polite

They were very fast extremely polite walked you through every detail of the process if you had any questions they had no problems answering

Billy Williams - 2/19/2025

Fast service and friendly staff!

Fast service and friendly staff! Could not recommend enough for drug testing services.

Habib Malik - 4/12/2025

Clear and Competent

I was so surprised with the quality of customer service I received today when I called Accredited Drug Testing. The representative was so clear and competent. She was able to reschedule an appointment at my preferred location. The representative whose name is Justice, was so polite and competent. She expedited the whole process. It was a relief to me.

Rachel Padian - 9/6/2024


(800) 221-4291