✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Frankfort, IL

With 37 testing centers in the Frankfort area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

37 Drug-Testing Centers in Frankfort

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

21035 S La Grange Rd

0.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
21035 S La Grange Rd
Frankfort, IL 60423

19070 Everett Blvd, Unit 111

3.36 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
19070 Everett Blvd, Unit 111
Mokena, IL 60448

Po Box 63

3.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Po Box 63
Mokena, IL 60448

18210 La Grange Rd Ste 110

5.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
18210 La Grange Rd Ste 110
Tinley Park, IL 60487

621 E Lincoln Hwy

5.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
621 E Lincoln Hwy
New Lenox, IL 60451

250 E Maple St

5.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
250 E Maple St
New Lenox, IL 60451

6701 159th St Occ Health

6.10 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6701 159th St Occ Health
Tinley Park, IL 60477

17148 Harlem Ave

6.45 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
17148 Harlem Ave
Tinley Park, IL 60477

10737 W. 165th Street

6.75 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 02:00 PM - 04:00 PM
10737 W. 165th Street
Orland Park, IL 60467

25711 S Egyptian Trail

7.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
25711 S Egyptian Trail
Monee, IL 60449

9570 W 159th St Ste A

7.31 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9570 W 159th St Ste A
Orland Park, IL 60467

16325 Harlem Ave Ste 110

7.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
16325 Harlem Ave Ste 110
Tinley Park, IL 60477

19550 Governors Hwy

8.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
19550 Governors Hwy
Flossmoor, IL 60422

19150 South Kedzie Avenue, Suite 101

8.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
19150 South Kedzie Avenue, Suite 101
Flossmoor, IL 60422

Onsite For Poh Only

8.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Onsite For Poh Only
Orland Park, IL 60462

14475 John Humphrey Dr Ste 100,

9.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14475 John Humphrey Dr Ste 100,
Orland Park, IL 60462

14330 S Will Cook Rd

9.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14330 S Will Cook Rd
Homer Glen, IL 60491

3301 N Ashland

10.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3301 N Ashland
Chicago, IL 60657

18665 Dixie Hwy

10.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
18665 Dixie Hwy
Homewood, IL 60430

16501 Kedzie Ave

10.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
16501 Kedzie Ave
Markham, IL 60428

1423 Chicago Rd

10.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1423 Chicago Rd
Chicago Heights, IL 60411

30 E 15th St Ste 100

10.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
30 E 15th St Ste 100
Chicago Heights, IL 60411

16221 W 159th St

11.12 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM • Sat:,Sun: 9:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
16221 W 159th St
Lockport, IL 60441

2025 S Chicago St

12.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2025 S Chicago St
Joliet, IL 60436

611 Division St

12.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
611 Division St
Peotone, IL 60468

12255 S 80th Ave

12.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12255 S 80th Ave
Palos Heights, IL 60463

4742 Cal Sag Rd

12.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4742 Cal Sag Rd
Crestwood, IL 60445

15600 Honore Ave

12.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
15600 Honore Ave
Harvey, IL 60426

1000 S Hamilton St

12.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1000 S Hamilton St
Lockport, IL 60441

7800 W College Dr, Room 1-w

12.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7800 W College Dr, Room 1-w
Palos Heights, IL 60463

4800 W 129th St

12.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4800 W 129th St
Alsip, IL 60803

2310 York St Ste 4a

14.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2310 York St Ste 4a
Blue Island, IL 60406

5533 W 109th St Ste 103

14.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5533 W 109th St Ste 103
Oak Lawn, IL 60453

800 N Larkin Ave

14.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
800 N Larkin Ave
Joliet, IL 60435

2000 Glenwood Ave

14.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2000 Glenwood Ave
Joliet, IL 60435

16101 Weber Rd

14.79 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
16101 Weber Rd
Crest Hill, IL 60403

310 N. Hammes Ave Ste 102,

14.80 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
310 N. Hammes Ave Ste 102,
Joliet, IL 60435

Employment, DOT, Court Ordered, Probation, Family

At 37 testing centers in Frankfort, Illinois, Accredited Drug Testing delivers extensive drug and alcohol testing services. We cater to both DOT and non-DOT requirements by offering urine and breath alcohol tests, EtG, and hair drug screenings for various purposes including personal, business, and legal. Our rapid testing services in Frankfort, IL offer prompt outcomes, with many testing spots conveniently located close to your residence or workplace. We also provide Occupational Health Testing, Clinical Testing, and Background Checks.

Contact us at (800) 221-4291 or register online by selecting a test and choosing a nearby location for yourself, your employees, or another person. Our testing procedure is straightforward—schedule tests through our department or use our online service available around the clock. Our efficient system simplifies arranging local drug tests, ensuring an easy experience in Frankfort.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Frankfort, IL

1

Order Online or Call

Select your test & search nearby Frankfort collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Frankfort, IL Drug Testing

Drug Testing Services Available at Our Frankfort, IL Labs:

At our Frankfort drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Illinois Drug Testing Laws & Compliance in Frankfort

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Frankfort, IL adhering strictly to 820 ILCS 55/1 et seq., federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
820 ILCS 55/1 et seq.
Medical Marijuana
Legal
Recreational Cannabis
Legal
Workplace Rights
Zero-Tolerance Protected

Illinois Cannabis Regulation and Tax Act upholds employer authority to enforce reasonable drug-free workplace policies, zero-tolerance rules, and safety testing.

Under 820 ILCS 55/1 et seq., employers in Frankfort may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal; Recreational: Legal in Illinois), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Frankfort, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Frankfort and Illinois.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Frankfort, IL

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Frankfort to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Frankfort Neighborhoods Covered:

● Downtown Frankfort ● Central Business District ● North Frankfort Commercial Corridor ● South Frankfort Industrial Park ● East Frankfort Metro Area ● West Frankfort Trade Center ● Medical & Healthcare District ● Airport / Logistics Corridor

Major Employment & Commercial Hubs Served:

Frankfort Main Street Commercial District • Frankfort Regional Trade Center • County Industrial Parkway • Metro Healthcare & Occupational Medical Corridor

Located anywhere in Frankfort? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Frankfort

Arbury Hills • Mokena • Frankfort Square • New Lenox • Orland Hills • Tinley Park • Matteson • Richton Park

Frankfort, IL Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Frankfort.

Test Type & Local Panel Detection Window Common Use Cases in Frankfort Turnaround Time
5-Panel Urine Screen in Frankfort →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Frankfort →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Frankfort →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Frankfort →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Frankfort →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Frankfort →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Frankfort, IL

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Frankfort.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Frankfort facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Frankfort, IL

Need immediate walk-in service in Frankfort? View our Frankfort, IL Drug Testing Facility Guide or order an instant electronic donor pass online.

Frankfort Drug Testing

Quickly find trusted local drug testing centers in Frankfort, IL — fast, convenient, and reliable every time!

Frankfort DOT Drug Testing

Quickly find a local DOT drug testing center in Frankfort, IL — fast, reliable, convenient nationwide service!

Frankfort DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Frankfort, IL.

Frankfort Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Frankfort, IL.v

Frankfort Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Frankfort, IL locations—results you can trust, every time!

Frankfort Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Frankfort, IL.

Frankfort Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Frankfort, IL.

Frankfort 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Frankfort, IL employers and individuals nationwide.

Frankfort 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Frankfort, IL workplace and personal testing needs.

Frankfort On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Frankfort, IL workplace compliant.

Frankfort DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Frankfort, IL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Illinois, workplace drug testing is governed by statutory guidelines under 820 ILCS 55/1 et seq.. Private employers generally operate under a Right to Privacy in the Workplace Act & Cannabis Regulation Act, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Illinois who implement a certified Drug-Free Workplace Program in compliance with 820 ILCS 55/1 et seq. can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Illinois state law compliance standards, state statutes protects registered patients while preserving workplace safety testing. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 37 certified collection sites in the Frankfort area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Frankfort, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

Very professional and very quick

Very professional and very quick service to schedule appointments! I am pleased with the services and would definitely recommend using them for any drug testing needs!

Carrie Feerick - 4/19/2025

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Explained everything so no questions

Torin was great. Explained everything so no questions

Tony Lombardo - 4/18/2025


(800) 221-4291