✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in San Antonio, FL

With 30 testing centers in the San Antonio area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

30 Drug-Testing Centers in San Antonio

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

13933 17th St Ste 101

4.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
13933 17th St Ste 101
Dade City, FL 33525

37802 Medical Arts Ct

7.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
37802 Medical Arts Ct
Zephyrhills, FL 33541

27421 State Road 54

7.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
27421 State Road 54
Wesley Chapel, FL 33544

7050 Gall Blvd

7.45 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7050 Gall Blvd
Zephyrhills, FL 33541

6719 Gall Blvd., Suite 101

7.63 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-2:00 PM • Sat: 7:30 AM-11:30 AM✓ Walk-in with Electronic Donor Pass
6719 Gall Blvd., Suite 101
Zephyrhills, FL 33542

6755 Gall Blvd

7.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6755 Gall Blvd
Zephyrhills, FL 33542

5219 10th St

9.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5219 10th St
Zephyrhills, FL 33542

5504 Gateway Blvd

9.38 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5504 Gateway Blvd
Wesley Chapel, FL 33544

1724 Bruce B Downs Blvd Ste Q2

11.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1724 Bruce B Downs Blvd Ste Q2
Wesley Chapel, FL 33544

26827 Foggy Creek Rd, Bldg. 6 Suite 102

11.76 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-2:00 PM • Sat: 7:30 AM-11:30 AM✓ Walk-in with Electronic Donor Pass
26827 Foggy Creek Rd, Bldg. 6 Suite 102
Wesley Chapel, FL 33544

20677 Bruce B Downs Blvd

12.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
20677 Bruce B Downs Blvd
Tampa, FL 33647

24420 State Road 54

13.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
24420 State Road 54
Lutz, FL 33559

31075 Cortez Blvd

13.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
31075 Cortez Blvd
Brooksville, FL 34602

34498 Cortez Blvd

13.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
34498 Cortez Blvd
Ridge Manor, FL 33523

22945 State Road 54

13.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
22945 State Road 54
Lutz, FL 33549

18101 Highwoods Preserve Pkwy

15.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
18101 Highwoods Preserve Pkwy
Tampa, FL 33647

20205 Cortez Blvd

15.84 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
20205 Cortez Blvd
Brooksville, FL 34601

11123 County Line Rd

17.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11123 County Line Rd
Spring Hill, FL 34609

40 Seven Hills Dr,

17.27 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
40 Seven Hills Dr,
Spring Hill, FL 34609

17240 Cortez Blvd

17.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
17240 Cortez Blvd
Brooksville, FL 34601

10441 Quality Dr Ste 105

17.72 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10441 Quality Dr Ste 105
Spring Hill, FL 34609

170 Mariner Blvd., Unit 7-a

18.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
170 Mariner Blvd., Unit 7-a
Spring Hill, FL 34609

3041 Landover Blvd.,

19.36 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-2:00 PM • Sat: 6:00 AM-10:00 AM✓ Walk-in with Electronic Donor Pass
3041 Landover Blvd.,
Spring Hill, FL 34608

4112 Mariner Blvd

19.47 mi
🕑 Testing Hours: Mon–Sat: 9:00 AM-9:00 PM • Sun: 10:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
4112 Mariner Blvd
Spring Hill, FL 34609

3037 Landover Blvd

19.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3037 Landover Blvd
Spring Hill, FL 34608

14438 University Cove Pl

20.10 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14438 University Cove Pl
Tampa, FL 33613

15511 N Florida Avenue, Suites 602

20.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
15511 N Florida Avenue, Suites 602
Tampa, FL 33613

12114 Cortez Blvd #205

20.46 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12114 Cortez Blvd #205
Brooksville, FL 34613

13610 Bruce B Downs Blvd

20.49 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
13610 Bruce B Downs Blvd
Tampa, FL 33613

3100 E Fletcher Ave

20.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3100 E Fletcher Ave
Tampa, FL 33613

Employment, DOT, Court Ordered, Probation, Family

Accredited Drug Testing delivers extensive drug and alcohol testing services from 30 centers around San Antonio, Florida. We conduct DOT, non-DOT urine drug screens, breath alcohol tests, EtG alcohol analysis, and hair drug exams for personal, workplace, and legal purposes. In San Antonio, FL, our rapid testing and SAMSA certified lab services offer swift results; same-day appointments can be scheduled, and most centers are conveniently located near your home or workplace. We also provide Occupational Health Testing, Clinical Testing, and Background Verifications.

Dial (800) 221-4291 or register through our online platform. Choose your preferred test and find a nearby center—testing services are ready for you, your employees, or others. Scheduling is a quick and simple process, either by contacting our scheduling team or by using our online service 24/7. Our efficient and accessible system makes setting up drug tests in San Antonio straightforward and hassle-free.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in San Antonio, FL

1

Order Online or Call

Select your test & search nearby San Antonio collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

San Antonio, FL Drug Testing

Drug Testing Services Available at Our San Antonio, FL Labs:

At our San Antonio drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Florida Drug Testing Laws & Compliance in San Antonio

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in San Antonio, FL adhering strictly to Fla. Stat. § 440.102, federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
Fla. Stat. § 440.102
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Prohibited
Workplace Rights
Zero-Tolerance Protected

Florida Statute 440.102 establishes Drug-Free Workplace Program standards, providing premium discounts on worker compensation insurance for verified testing procedures.

Under Fla. Stat. § 440.102, employers in San Antonio may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Prohibited in Florida), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in San Antonio, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout San Antonio and Florida.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in San Antonio, FL

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout San Antonio to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key San Antonio Neighborhoods Covered:

● Downtown San Antonio ● Central Business District ● North San Antonio Commercial Corridor ● South San Antonio Industrial Park ● East San Antonio Metro Area ● West San Antonio Trade Center ● Medical & Healthcare District ● Airport / Logistics Corridor

Major Employment & Commercial Hubs Served:

San Antonio Main Street Commercial District • San Antonio Regional Trade Center • County Industrial Parkway • Metro Healthcare & Occupational Medical Corridor

Located anywhere in San Antonio? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near San Antonio

Saint Leo • St. Leo • Pasadena Hills • Dade City North • Blanton • Wesley Chapel South • Central Pasco • Wesley Chapel

San Antonio, FL Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in San Antonio.

Test Type & Local Panel Detection Window Common Use Cases in San Antonio Turnaround Time
5-Panel Urine Screen in San Antonio →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in San Antonio →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in San Antonio →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in San Antonio →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in San Antonio →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in San Antonio →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in San Antonio, FL

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across San Antonio.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your San Antonio facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in San Antonio, FL

Need immediate walk-in service in San Antonio? View our Walk-In Drug Testing Near Me in San Antonio, FL facility guide or order an instant electronic donor pass online.

San Antonio Drug Testing

Quickly find trusted local drug testing centers in San Antonio, FL — fast, convenient, and reliable every time!

San Antonio DOT Drug Testing

Quickly find a local DOT drug testing center in San Antonio, FL — fast, reliable, convenient nationwide service!

San Antonio DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around San Antonio, FL.

San Antonio Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in San Antonio, FL.v

San Antonio Hair Drug Testing

Get fast, accurate hair drug testing at our convenient San Antonio, FL locations—results you can trust, every time!

San Antonio Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in San Antonio, FL.

San Antonio Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in San Antonio, FL.

San Antonio 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by San Antonio, FL employers and individuals nationwide.

San Antonio 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for San Antonio, FL workplace and personal testing needs.

San Antonio On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your San Antonio, FL workplace compliant.

San Antonio DOT Physicals

Certified DOT physicals with quick, reliable exams to keep San Antonio, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Florida, workplace drug testing is governed by statutory guidelines under Fla. Stat. § 440.102. Private employers generally operate under a Drug-Free Workplace Program under Fla. Stat. § 440.102, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Florida who implement a certified Drug-Free Workplace Program in compliance with Fla. Stat. § 440.102 can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Florida state law compliance standards, state statutes does not require accommodation of medical marijuana in workplace. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 30 certified collection sites in the San Antonio area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across San Antonio, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

Smooth and Seamless

I use their service for new hire and DOT employee's. Spoke with Taisha Walker this morning, and she was very helpful. She made the process smooth and seamless.

Christina Galdos - 3/9/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

Super helpful

Customer service was surprisingly super helpful and so nice. She was able to get me in at a local location right away the same day. She helped me figure out exactly what I needed. Very impressed.

Brook - 9/19/2024

Clear and Competent

I was so surprised with the quality of customer service I received today when I called Accredited Drug Testing. The representative was so clear and competent. She was able to reschedule an appointment at my preferred location. The representative whose name is Justice, was so polite and competent. She expedited the whole process. It was a relief to me.

Rachel Padian - 9/6/2024

Saved My CDL!

Time was running out before my Cdl got downgraded because of a violation I had on clearinghouse. I couldn't find an employer to send me for my return to duty test, but these guys had my test scheduled and done in the same day! They saved my cdl. Thank you again!

Michael Williams - 12/2/2024

Kind and very helpful

Called around for hours and posted from company to person and got nowhere and had to fight to get a refund die to there miss information, well save time and sanity Acredited is the ONLY place the person was kind and very helpful, said what I needed and Faith the lady I dealt with made it simple and she did above and beyond everything I needed, as I'm going through a devorse this was a great help and made my day 100 stars from me.

Steven Harrison - 12/19/2024

Had very fast and good service

Had very fast and good service, was able to get an appointment same day with the nearest facility. My questions were answered thoroughly but quick. Definitely recommend

Jacob Matzke - 11/19/2024

Super Easy!

Super easy to set up an appointment through the phone. They walked me through all the steps and found me a location right near my house. Would definitely recommend!

Aldita Lumaj - 1/17/2025

I love the customer service

I love the customer service. The lady was patient enough to help me book my appointment with ease and ensuring I get the location that's best for me. Keep up the good work!

Elizabeth Chiaka - 9/19/2024

phone support has been excellent

I have used ADT several times. Their phone support has been excellent, both when putting in the orders and when asking for help to interpret results.

Tee Ess - 12/19/2024


(800) 221-4291