✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Wesley Chapel, FL

With 36 testing centers in the Wesley Chapel area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

36 Drug-Testing Centers in Wesley Chapel

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

27421 State Road 54

0.18 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
27421 State Road 54
Wesley Chapel, FL 33544

5504 Gateway Blvd

2.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5504 Gateway Blvd
Wesley Chapel, FL 33544

1724 Bruce B Downs Blvd Ste Q2

4.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1724 Bruce B Downs Blvd Ste Q2
Wesley Chapel, FL 33544

26827 Foggy Creek Rd, Bldg. 6 Suite 102

4.41 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-2:00 PM • Sat: 7:30 AM-11:30 AM✓ Walk-in with Electronic Donor Pass
26827 Foggy Creek Rd, Bldg. 6 Suite 102
Wesley Chapel, FL 33544

20677 Bruce B Downs Blvd

5.10 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
20677 Bruce B Downs Blvd
Tampa, FL 33647

24420 State Road 54

6.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
24420 State Road 54
Lutz, FL 33559

22945 State Road 54

7.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
22945 State Road 54
Lutz, FL 33549

18101 Highwoods Preserve Pkwy

7.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
18101 Highwoods Preserve Pkwy
Tampa, FL 33647

37802 Medical Arts Ct

8.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
37802 Medical Arts Ct
Zephyrhills, FL 33541

6719 Gall Blvd., Suite 101

8.51 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-2:00 PM • Sat: 7:30 AM-11:30 AM✓ Walk-in with Electronic Donor Pass
6719 Gall Blvd., Suite 101
Zephyrhills, FL 33542

6755 Gall Blvd

8.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6755 Gall Blvd
Zephyrhills, FL 33542

7050 Gall Blvd

8.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7050 Gall Blvd
Zephyrhills, FL 33541

5219 10th St

9.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5219 10th St
Zephyrhills, FL 33542

13933 17th St Ste 101

11.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
13933 17th St Ste 101
Dade City, FL 33525

14438 University Cove Pl

12.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14438 University Cove Pl
Tampa, FL 33613

15511 N Florida Avenue, Suites 602

12.90 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
15511 N Florida Avenue, Suites 602
Tampa, FL 33613

13610 Bruce B Downs Blvd

13.09 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
13610 Bruce B Downs Blvd
Tampa, FL 33613

3100 E Fletcher Ave

13.13 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3100 E Fletcher Ave
Tampa, FL 33613

11315 N 30th St

13.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11315 N 30th St
Tampa, FL 33612

10320 N 56th St Ste 110

14.19 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
10320 N 56th St Ste 110
Temple Terrace, FL 33617

15423 N Dale Mabry Hwy Ste 101

14.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
15423 N Dale Mabry Hwy Ste 101
Tampa, FL 33618

3837 Northdale Blvd Ste 140

14.84 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3837 Northdale Blvd Ste 140
Tampa, FL 33624

3450 Bushwood Park Dive Ste 100

15.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3450 Bushwood Park Dive Ste 100
Tampa, FL 33618

13856 N Dale Mabry Hwy

15.90 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
13856 N Dale Mabry Hwy
Tampa, FL 33618

1010 E Busch Blvd Ste 103

16.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1010 E Busch Blvd Ste 103
Tampa, FL 33612

11752 E Dr Martin Luther King Jr Blvd

17.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11752 E Dr Martin Luther King Jr Blvd
Seffner, FL 33584

801 W Dr Martin Luther King Jr Blvd

17.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
801 W Dr Martin Luther King Jr Blvd
Seffner, FL 33584

6182 Gunn Hwy

18.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6182 Gunn Hwy
Tampa, FL 33625

11123 County Line Rd

18.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11123 County Line Rd
Spring Hill, FL 34609

40 Seven Hills Dr,

18.47 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
40 Seven Hills Dr,
Spring Hill, FL 34609

3110 Cherry Palm Dr

18.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3110 Cherry Palm Dr
Tampa, FL 33619

3012 N Us Highway 301

18.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3012 N Us Highway 301
Tampa, FL 33619

10441 Quality Dr Ste 105

18.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10441 Quality Dr Ste 105
Spring Hill, FL 34609

4602 N Nebraska Ave

19.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4602 N Nebraska Ave
Tampa, FL 33603

170 Mariner Blvd., Unit 7-a

19.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
170 Mariner Blvd., Unit 7-a
Spring Hill, FL 34609

7001 N Dale Mabry Hwy Ste 10

19.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7001 N Dale Mabry Hwy Ste 10
Tampa, FL 33614

Employment, DOT, Court Ordered, Probation, Family

At our 36 facilities in Wesley Chapel, Florida, Accredited Drug Testing delivers thorough drug and alcohol testing services. We cater to DOT and non-DOT requirements with urine and breath alcohol tests, EtG for alcohol, and hair analysis, suitable for personal, employment, or legal purposes. Our Wesley Chapel, FL centers offer quick test results with SAMSA-certified labs, providing same-day services—all within convenient reach from home or office. We also provide Occupational Health Testing, Clinical Diagnostics, and Background Verification.

Contact us at (800) 221-4291 or opt for online registration. Choose your preferred test and select a location nearby—you can arrange testing for yourself, your workforce, or others. The scheduling process is rapid and uncomplicated; reach our department by phone or secure your appointment online round the clock. Our efficient system facilitates easy drug test arrangements near Wesley Chapel.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Wesley Chapel, FL

1

Order Online or Call

Select your test & search nearby Wesley Chapel collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Wesley Chapel, FL Drug Testing

Drug Testing Services Available at Our Wesley Chapel, FL Labs:

At our Wesley Chapel drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Florida Drug Testing Laws & Compliance in Wesley Chapel

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Wesley Chapel, FL adhering strictly to Fla. Stat. § 440.102, federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
Fla. Stat. § 440.102
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Prohibited
Workplace Rights
Zero-Tolerance Protected

Florida Statute 440.102 establishes Drug-Free Workplace Program standards, providing premium discounts on worker compensation insurance for verified testing procedures.

Under Fla. Stat. § 440.102, employers in Wesley Chapel may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Prohibited in Florida), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Wesley Chapel, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Wesley Chapel and Florida.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Wesley Chapel, FL

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Wesley Chapel to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Wesley Chapel Neighborhoods Covered:

● Downtown Wesley Chapel ● Central Business District ● North Wesley Chapel Commercial Corridor ● South Wesley Chapel Industrial Park ● East Wesley Chapel Metro Area ● West Wesley Chapel Trade Center ● Medical & Healthcare District ● Airport / Logistics Corridor

Major Employment & Commercial Hubs Served:

Wesley Chapel Main Street Commercial District • Wesley Chapel Regional Trade Center • County Industrial Parkway • Metro Healthcare & Occupational Medical Corridor

Located anywhere in Wesley Chapel? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Wesley Chapel

Wesley Chapel South • Central Pasco • Pebble Creek • Pasadena Hills • San Antonio • Land O' Lakes • Saint Leo • Pasco

Wesley Chapel, FL Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Wesley Chapel.

Test Type & Local Panel Detection Window Common Use Cases in Wesley Chapel Turnaround Time
5-Panel Urine Screen in Wesley Chapel →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Wesley Chapel →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Wesley Chapel →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Wesley Chapel →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Wesley Chapel →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Wesley Chapel →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Wesley Chapel, FL

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Wesley Chapel.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Wesley Chapel facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Wesley Chapel, FL

Need immediate walk-in service in Wesley Chapel? View our Walk-In Drug Testing Near Me in Wesley Chapel, FL facility guide or order an instant electronic donor pass online.

Wesley Chapel Drug Testing

Quickly find trusted local drug testing centers in Wesley Chapel, FL — fast, convenient, and reliable every time!

Wesley Chapel DOT Drug Testing

Quickly find a local DOT drug testing center in Wesley Chapel, FL — fast, reliable, convenient nationwide service!

Wesley Chapel DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Wesley Chapel, FL.

Wesley Chapel Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Wesley Chapel, FL.v

Wesley Chapel Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Wesley Chapel, FL locations—results you can trust, every time!

Wesley Chapel Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Wesley Chapel, FL.

Wesley Chapel Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Wesley Chapel, FL.

Wesley Chapel 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Wesley Chapel, FL employers and individuals nationwide.

Wesley Chapel 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Wesley Chapel, FL workplace and personal testing needs.

Wesley Chapel On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Wesley Chapel, FL workplace compliant.

Wesley Chapel DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Wesley Chapel, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Florida, workplace drug testing is governed by statutory guidelines under Fla. Stat. § 440.102. Private employers generally operate under a Drug-Free Workplace Program under Fla. Stat. § 440.102, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Florida who implement a certified Drug-Free Workplace Program in compliance with Fla. Stat. § 440.102 can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Florida state law compliance standards, state statutes does not require accommodation of medical marijuana in workplace. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 36 certified collection sites in the Wesley Chapel area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Wesley Chapel, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

Super helpful and courteous

I've had to use this service twice for out of state physicians we've hired and both times it was super easy. Both customer service reps I spoke with were super helpful and courteous. I won't hesitate to use their service again if needed.

Alicia Rau - 6/19/2024

test was quick and easy

Company New Hire Employee Process for DOT Drug test was quick and easy. No need to make appt. All employee had to do is show prepaid qr code I sent to employee show ID and was set. Would Recommend.

Maria Lopez - 11/19/2024

Polite

I ran into a situation because the Guthrie had closed and I had to go to well care and they were right on it. I had no problems when I called to get connected with welcare everything worked out and I didn't even have to wait.they were polite and talked with the receptionist for me once I got them on the phone.

Sayre - 8/19/2024

Super helpful

Customer service was surprisingly super helpful and so nice. She was able to get me in at a local location right away the same day. She helped me figure out exactly what I needed. Very impressed.

Brook - 9/19/2024

Clear and Competent

I was so surprised with the quality of customer service I received today when I called Accredited Drug Testing. The representative was so clear and competent. She was able to reschedule an appointment at my preferred location. The representative whose name is Justice, was so polite and competent. She expedited the whole process. It was a relief to me.

Rachel Padian - 9/6/2024

Great service

Great service easy use quick processing! Need a hair follicle for my DCFS case , quick and convenient!

Jesse Dupre - 4/12/2025

Super Easy!

Super easy to set up an appointment through the phone. They walked me through all the steps and found me a location right near my house. Would definitely recommend!

Aldita Lumaj - 1/17/2025

Very helpful

They were very helpful in finding a place to get tested on the route back home! They made a difficult time seem easy with no judgement!

Anne Creden - 11/19/2024

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

Reception is kind and helpful

Reception is kind and helpful , everything quick and simple , best urgent care

Yusuf Narmuradov - 11/19/2024


(800) 221-4291